Fig. 4. Structure of the SALMFamide precursor gene in Strongylocentrotus purpuratus. The DNA sequence of the gene is shown in lowercase letters with base positions in contig 347664 shown in the column on the right (11762-13920). The predicted precursor sequence is shown in uppercase letters with amino acid positions shown in bold and in brackets in the column on the right (1-266). The gene comprises two exons, whose positions are indicated by the underlying amino acid sequence in uppercase letters, with the start and end of the intervening intron labelled in bold letters (gt/ag). 24 bases upstream of the start codon (atg) in the first exon is a putative TATA-box-like promoter sequence (tttatt), which is shown in bold. The predicted signal peptide sequence encoded by the first exon is shown in underlined italics, based on the analysis shown in Fig. 3B. Amino acid sequences corresponding to putative SALMFamide neuropeptides encoded by the second exon are shown underlined, with monobasic and dibasic (K, KR, RR) cleavage sites shown in bold. In the 3' region of the gene there is consensus polyadenylation signal sequence (aataaa) located 47 bases downstream from the stop codon (^*).