Fig. 3. The effects of L-Arg and L-NAME supplementation and different temperatures on UCP1 protein level in IBAT. (A) Western blotting was performed using specific antibody for UCP1. The results of three observations from a representative experiment and the position of molecular mass markers (kDa) are shown. C, control. (B) Data obtained after quantification of UCP1 bands by ImageQuant software. Relative UCP1 concentration was expressed in relation to the control cold-acclimated group taken as 100%. The values represent the means ± S.E.M. from three independent experiments. Comparison of different treatments with the control kept at the same temperature, P<0.005. Volume is the sum of all pixel intensities within a band; 1 pixel=0.007744 mm².