Fig. 2. The effects of L-Arg and L-NAME supplementation and different temperatures (±1°C) on the iNOS protein level in IBAT. (A) Western blotting was performed using specific antibody against iNOS. The results of three observations from a representative experiment and the position of molecular mass markers (kDa) are shown. C, control. (B) Data obtained after quantification of iNOS bands by ImageQuant software. iNOS concentration is expressed in relative to the levels in controls acclimated to room temperature (taken as 100%). The values represent the means ± S.E.M. from three independent experiments. ^*Comparison of the same treatments at different temperatures, ^**P<0.025; ^***P<0.005; comparison of different treatments with the control kept at the same temperature, P<0.025; P<0.005. Volume is the sum of all the pixel intensities within a band; 1 pixel=0.007744 mm².