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Fig. 5. MHC isoform expression in bovine laryngeal muscles. (A) RT-PCR using specific primers for MHC-2B, MHC-Eo and MHC-Neo. (B) Electrophoretic separation of MHC isoforms; the migration order is (from top to bottom): MHC-2A, MHC-Emb, MHC-2X and MHC-Neo (comigration), MHC-2B, MHC-1. Cd, cricoarytenoideus dorsalis; At, arytenoideus transverses; T, thyreoarytenoideus [Tvr, Tvc (pars rostralis and caudalis of ventricular part) and Tvo (pars vocalis)]; Rb, retractor bulbi (used as a control; note that MHC-1 is not detectable in this sample).