Fig. 1. Hsp70 expression in honey bee nurses and foragers. (A,B) Hsp70 expression in heads; (C,D) Hsp70 expression in thoraces. Hsp70 family proteins were isolated on western blots labeled with a Hsp70 mouse monoclonal antibody (Sigma, St Louis, MO, USA; H5147) and an anti-mouse secondary antibody conjugated to horseradish peroxidase (Sigma). Proteins were visualized with enhanced chemiluminescence and quantified on a Typhoon Phosphorimager (Amersham, UK). The Hsp70 standard is 0.5 µg of purified Hsp70 protein (from bovine brain; Sigma; H9776). Numbers in bars indicate sample size.