Fig. 9. Semi-quantitative analysis of msslo mRNA developmental profile in skeletal muscle confirms regional-specific expression patterns. Densitometric analysis using GeneTools software revealed (A) similar and low levels of msslo transcripts in larval and adult abdominal DLMs compared with early-stage pupae. (B) The dramatic increase in msslo expression in thoracic DLMs is in contrast to the abdominal pattern. Semi-quantification of the chemiluminescence was done by comparing the intensity of the msslo mRNA band with that of the internal control, rpS3. Mean and S.E.M. are plotted (N=5). Abbreviations: day 2 fifth instar larvae (V2), day 6 after pupation (P6) and pharate adults (PA).