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Fig. 7. Semi-quantitative analysis of msslo mRNA developmental profile in visceral muscle. Densitometric analysis of heart (A) and midgut (B) northern blots using GeneTools software confirmed muscle-specific developmental profiles. (A) In the heart, msslo transcript levels transiently decline during development whereas (B) in the midgut, levels are low until the adult stage. Semi-quantification of the chemiluminescence was done by comparing the intensity of the msslo mRNA band with that of the internal control, rpS3. Mean and S.E.M. are plotted (N=3). Abbreviations: day 2 fifth instar larvae (V2), day 6 after pupation (P6) and pharate adults (PA).