Fig. 6. Muscle-specific developmental changes in msslo expression occur in the heart and midgut. (A) In the heart, msslo mRNA expression appears to transiently decline in pupae from larval levels and then return to similar expression levels in pharate adults. (B) In contrast to the heart, midgut levels of msslo mRNA are highest in pharate adults. Total RNA (5 µg) of day 2 fifth instar larvae (V2), day 6 pupae (P6) and pharate adults (PA) was hybridized to the digoxigenin-labeled msslo 680 bp cDNA insert. For the loading control, the lower portion of the blots was hybridized to the digoxigenin-labeled M. sexta ribosomal protein S3 (rpS3) cDNA insert (Jiang et al., 1996). The sizes (kb) of the hybridizing RNAs are shown on the left.