Fig. 5. Semi-quantitative analysis of msslo mRNA developmental profile within the CNS. Densitometric analysis of CNS northern blots using GeneTools software showed relatively constant levels of msslo transcripts throughout larval life, with levels fluctuating during the larval-pupal transition and increasing in adults. Semi-quantification of the chemiluminescence was done by comparing the intensity of the msslo mRNA band with that of the internal control, rpS3. Mean and S.E.M. are plotted (N=5). Abbreviations: day 2 fifth instar larvae (V2), days 0 and 3 wandering larvae (W0, W3), days 0, 2, 4, 6, 8, 10, 12, 14 after pupation (P0, P2, P4, P6, P8, P10, P12, P14) and pharate adults (PA).