Fig. 4. Developmental profile of msslo mRNA within the CNS. No dramatic changes in msslo gene expression are detected within the CNS during metamorphosis. Total RNA (1 µg per lane) was isolated from day 2 fifth instar larvae (V2), days 0 and 3 wandering larvae (W0, W3), days 0, 2, 4, 6, 8, 10, 12, 14 after pupation (P0, P2, P4, P6, P8, P10, P12, P14) and pharate adults (PA) and hybridized to the digoxigenin-labeled msslo 680 bp cDNA insert or loading control M. sexta ribosomal protein S3 (rpS3) cDNA insert (Jiang et al., 1996). The sizes (kb) of the hybridizing RNAs are shown on the left. The major mRNA is 4 kb and is similar in size to the msslo cDNA. The 11 kb band is probably pre-processed msslo mRNA.