Fig. 2. Deduced amino sequence of msSlo and its alignment to insect and mammalian Slowpoke proteins. The sequences were compared using AlignX software (Informax, Inc.). The black shading indicates identical residues; the gray shading indicates similar residues. The dashes indicate gaps in the alignment. The approximate location of the seven membrane-spanning regions (S0-S6), the pore region (P), the four hydrophobic C-terminal cytoplasmic regions (S7-S10) and the calcium bowl are marked by black bars. The beginning of the five alternate splice sites (A, C, E, G, I) found in the insect sequences are marked with an arrowhead. GenBank accession numbers for sequences: Manduca sexta Slo (AY644784), Drosophila melanogaster Slo (M96840), Periplaneta americana Slo (AF452164), Mus musculus Slo (NM010610). Abbreviations: dSlo, Drosophila melanogaster Slowpoke; msSlo, Manduca sexta Slowpoke; pSlo, Periplaneta americana Slowpoke; mSlo, mouse Slowpoke.