Fig. 11. Temporal correlation of changes in msslo expression with the development of the dorsal longitudinal flight muscle. The dramatic increase in msslo mRNA occurs late in adult development, preceding the muscle's competence to respond to the mature flight motor pattern. The slight increase in message at P8 precedes the first time DLM muscle potentials can be recorded and the onset of maturation of the flight motor pattern within the CNS. Semi-quantification of msslo mRNA was done by comparing its signal intensity to the signal intensity of the housekeeping gene rpS3 using image analysis software (GeneTools). Mean and S.E.M. are plotted (N=5). Developmental events based on Kammer and Kinnamon (1979), Rheuben and Kammer (1980), Duch et al. (2000) and Duch and Levine (2002).