Fig. 9. Enhanced production of reactive oxygen species in G. dibranchiata
erythrocytes exposed to sulfide (up to 1.9 mmol l-1) for 1 h. (A)
Formation of fluorescent 2',7'-dichlorofluorescein (DCF) from
nonfluorescent 2',7'-dichlorodihydrofluorescein diacetate
(H2DCFDA) in erythrocytes (closed circles) or incubation buffer
alone (open circles). DCF fluorescence is normalized to DCF formation in
erythrocytes under control conditions (no sulfide). (B) Oxidation of
MitoSOXTM Red in erythrocytes. Fluorescence is normalized to control
conditions (no sulfide). Values are means ± S.D.
(N=4). Asterisks represent a significant effect of sulfide compared
to the control value (no sulfide) by RM ANOVA
(***P<0.005).