Fig. 5. TMRM fluorescence in G. dibranchiata erythrocytes exposed to sulfide (0.3, 0.5, 0.8 and 1.2 mmol l^-1) for 1 h followed by recovery in sulfide-free buffer for 2 or 5 h. Fluorescence emission (595 nm) at each time point is normalized to the emission of control erythrocytes (sulfide-free buffer) at the same time point. Values are means ± S.D. (N=3). Statistical analyses were performed by two-factor, RM ANOVA followed by Fisher's LSD post-hoc test (note that this is less conservative than the Tukey HSD test). Asterisks adjacent to data points indicate a significant difference from the control value (no sulfide) at the same time point (^*P<0.05, ^**P<0.01, ^***P<0.005); daggers indicate a significant main effect of sulfide at that concentration (P<0.05, P<0.01, P<0.005). Note that there was no significant effect of time (P=0.83) and no significant interaction between sulfide and time (P=0.88).