Fig. 7. An abrupt slackening in the membrane vesicle (Ai) derived from the contractile vacuole complex and the contractile vacuole (Bi) from P. multimicronucleatum cells. (Aii, Bii) Time course of change in the area of the image of the vesicle and the CV, respectively. Areas are presented as the percentage of the largest area achieved during the maximum degree of slackening prior to CV pore opening during the recording. Insets in Aii and Bii are expanded portions of the time courses presented in red. See the text for the details. Scale bars, 50 µm.