Fig. 3. Changes in the input resistance and the membrane potential of a membrane vesicle derived from the contractile vacuole complex of a P. multimicronucleatum cell accompanied by images of the vesicle's rounding-slackening. (Ai, Bi) Frames of two different vesicles showing rounding and slackening. Rounding of the vesicle is recognizable by the enhanced shadow effect (maximum rounding in Ai is in e and in Bi is in g before bursting in h). (Aii, Bii) Time courses of change in the input resistance, R_m, for the vesicles. (Aiii, Biii) Time courses of change in the membrane potential, V_m, for the vesicles. (a–h) in Ai and Bi correspond to the sequence when each picture was taken, which is also indicated by short vertical bars marked by the letters on the traces for R_m (Aii, Bii) and V_m (Aiii, Biii). e, patch-clamp electrode; n, microneedle; v, membrane vesicle. Scale bars, 50 µm.