Fig. 1. Two series of pictures of the contractile vacuole complex-derived membrane vesicles obtained from two different P. multimicronucleatum cells (Ai, Bi). (Aii, Aiii, Bii) The I–V relationships for different membrane vesicles in Ai and Bi. Ai, Bi reveal the portion of the contractile vacuole complex from which each vesicle is derived. n, a microneedle used for incision of the cell or for preventing fusion of the membrane vesicles; p, suction pipette for removing solution around the cell; e, patch-clamp electrode. Scale bars, 50 µm. See the text for details.