Fig. 9. Quantitative immunoblotting of the membrane fraction of gills of sham-operated, NaCl-, acid- and base-infused fish. (A) representative immunoblot incubated with anti-NHE2 antibody, showing a distinct band at 80 kDa. (B) Fluorometric analysis showing that the abundance of NHE2 in acid infused fish was 213±5% of sham-operated fish (100±21%). N=4; ^*P<0.05 (one-way ANOVA, Dunnet's post test).