Fig. 3. Cellular damage in response to experimental hyperoxia. (A) Carbonyl and (B)
MDA contents were measured in the three compartments of A. viridis
(Ec, ectoderm; Ed, endoderm; FIZ, freshly isolated zooxanthellae) during the
daytime. (C) Carbonyls and (D) MDA content were also measured in A.
schmidti total extract during the daytime, for control (C) and
hyperoxia-treated specimens (H). Cytosolic fractions containing a quantity of
100 and 150 µg of protein were analyzed for carbonyl and MDA content,
respectively. Data are presented as means ± S.E.M. of four
independent analyses. *Indicates significant differences between
control and stressed specimens (Student's t-test
P<0.05).