Fig. 5. Determination of Agdsx gene copy number in male and female genomic DNA. Genomic DNA (5 µg) extracted from male and female A. gambiae was digested with XhoI, HindIII and BamHI and electrophoresed on a 1% agarose gel. Hybridization was performed with a radiolabeled Agdsx Probe 5' that has no restriction site recognized by the three restriction enzymes. The molecular size is shown in kb.