Fig. 2. RT-PCR and northern blot analyses of total A. gambiae RNA. (A-F) RT-PCR products were amplified from female and male adult A. gambiae total RNA using the following combinations of primers: (A) dsx1f and dsx1r; (B) dsx2f and dsx1r; (C) dsx1f and dsx2r; (D) dsx3f and dsx3r; (E) dsxef and dsxer; (F) S7for and S7rev (which amplify the housekeeping ribosomal S7 gene as a control). For primer locations, see Fig. 1. (G) Northern blot analysis was performed on total RNA (10 µg) extracted from male and female A. gambiae adults. Lane 1, female adult; lane 2, male adult. The upper panel shows hybridization performed with Probe 5', while the lower panel shows standardization with probe S7. The molecular size is shown in kb.