Fig. 5. Quantitative PCR analysis of mRNA transcript abundance in anterior (3, 4,
5) and posterior (6, 7, 8) gills of Chasmagnathus granulatus
following transfer from 30
salinity to 45
, normalized to the
mean of posterior gills from crabs acclimated to 30
seawater
(zero-time value). (A) Arginine kinase; (B) V-type H+-ATPase
B-subunit; (C) Na+/K+/2Cl- cotransporter; (D)
Na+/K+-ATPase
-subunit. Means and standard errors
were calculated from gills 3, 4 and 5 (anterior) and gills 6, 7 and 8
(posterior), with triplicate determinations for each gill preparation. Results
of statistical analysis by ANOVA and post hoc comparisons are
presented in the text.