Fig. 1. Amplification of target transporter and housekeeping cDNAs from
Chasmagnathus granulatus gill by conventional PCR. Expected sizes of
amplification products are indicated in parentheses: lane 1, DNA ladder; lane
2, arginine kinase (1088 bp); lane 3, DNA ladder; lane 4, V-type
H+-ATPase B-subunit (390 bp); lane 5,
Na+/K+/2Cl- cotransporter (2100 bp); lane 6,
Na+/K+-ATPase
-subunit (703 bp); lane 7, DNA
ladder. Sizes of ladder standards are indicated at the right; each gel image
was adjusted to give parallel ladder bands.