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Fig. 1. Amplification of target transporter and housekeeping cDNAs from Chasmagnathus granulatus gill by conventional PCR. Expected sizes of amplification products are indicated in parentheses: lane 1, DNA ladder; lane 2, arginine kinase (1088 bp); lane 3, DNA ladder; lane 4, V-type H+-ATPase B-subunit (390 bp); lane 5, Na+/K+/2Cl- cotransporter (2100 bp); lane 6, Na+/K+-ATPase {alpha}-subunit (703 bp); lane 7, DNA ladder. Sizes of ladder standards are indicated at the right; each gel image was adjusted to give parallel ladder bands.