Fig. 11. Recombinant escapin and its antibacterial activity. (A) Western blots demonstrating successful expression of recombinant escapin. (B) Plate assay of inhibition of growth of E. coli by wild-type escapin and recombinant escapin. E. coli cells in stationary phase were grown at 37°C as a lawn on LB solid medium and tested against wild-type escapin and recombinant escapin at 7-60 µg ml^-1. Concentrations of wild-type and recombinant escapin were estimated from western blots. WEsc, wild-type escapin purified from ink; r-proEsc, recombinant escapin, with 18 amino acid signal peptide, in E. coli cell lysate; rEsc, recombinant in E. coli cell lysate, escapin without signal sequence (i.e. lacking 18 amino acids at N terminus); M, molecular mass markers.