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Fig. 7. (A) An example of a 2D-DIGE gel obtained by combining samples of normoxic and hypoxic Hb on the same gel. The gel was scanned at the appropriate wavelength for each fluor. Top panel, combined scan; lower two panels are the separated scans. (B) An example of DeCyder analysis of spots. Areas outlined in blue represent an increase of at least 2.5 times normoxic values. The spot enclosed in pink is the specific area being analyzed by DeCyder software. The lower panels demonstrate the difference in volume between the spot on the normoxic vs the hypoxic gel.