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Fig. 6. Two-dimensional gel electrophoresis of the hypoxic induction of Triops Hb. Normoxic-reared (NR), hypoxic transfer day 2 and 4 (HTD2, HTD4) and hypoxic-reared (HR). A train of spots corresponded with the molecular masses of Hb{alpha} and Hb{delta}; therefore, more than one of the spots was removed and sequenced for comparison. The lettered circled spots were selected for NH2-terminal amino acid sequencing.