Fig. 7. The effects of CabTRP Ia on nerve-evoked excitatory junction potentials
(EJPs) in six stomatogastric muscles. (A) Nerve-evoked EJP in a gastric mill
8a (gm8a) muscle fiber in control saline (black), after 10 min in
107 mol l1 CabTRP Ia (red), and after
rinsing the peptide (green). The membrane potential was 77 mV for all
three conditions. Each trace in this panel is the average of four EJPs
elicited at 20 s intervals. (B) A bar graph plot of average gm8a EJP amplitude
in control saline and after 10 min in 107 mol
l1 CabTRP Ia. The increase in amplitude in CabTRP Ia was
significant (N=6; paired t-test, **P<0.01). (C)
A bar graph showing the average increase in EJP amplitude in
107 mol l1 CabTRP Ia for six
stomatogastric muscles: gastric mill 4 (gm4; N=5), gastric mill 5a
(gm5a; N=6), gastric mill 6a (gm6a; N=8), gm8a
(N=6), pyloric 1 (p1; N=8) and pyloric 2 (p2; N=6).
For each individual experiment used to construct this panel, the EJP amplitude
was determined by averaging four EJPs, as in A. For each muscle, significance
was computed using paired t-tests on the EJP amplitudes
(*P<0.05, **P<0.01, ***P<0.001).