Fig. 4. Treatment of Ae. aegypti adult female posterior midgut with MTZ and ACZ combined (10^–4 mol l^–1 each) resulted in a significant decrease of pH inside the midgut (left) when compared with those midguts dissected from mosquitoes not treated with these inhibitors (right), when compared with the Cresol Red standards (bottom). (A) Dissected midgets. (B) Intact mosquitoes. (C) Dissected midguts photographed at 10x. Results shown are representative of 10–20 treated and untreated individuals from triplicate experiments.