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Fig. 1. The pH inside the adult Ae. aegypti mosquito midgut. Adult females were fed a mixture containing 0.02% pH indicator. (A) Picture of the dissected mosquito midguts under a stereoscopic microscope. (B) Ae. aegypti females immediately after feeding on the indicator mixture. (C) Dissected midguts of engorged mosquitoes photographed at 10x. (D) pH standards obtained using Cresol Red, Phenol Red, Neutral Red and Thymol Blue. Our results indicate that the pH inside the posterior midgut in adult females from Ae. aegypti ranges between 8.5 and 9.5.