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Fig. 1. (A) Schematic diagram of the custom-built chamber used for studies of sporozoite chemotaxis. a, 18 mmx18 mm coverglass; b, 9 mmx18 mm coverglass. (B) Diagram of the mathematical procedure used to analyze the direction of sporozoite movement calculated as tangency angle {theta}. Movement directed to the bottom of the figure is defined as 90°.