Fig. 9. Nucleotide and deduced amino acid sequence of partial cDNA encoding ecdysone receptor (Gl-EcR) from land crab. The cDNA sequence (1005 bp) encoded a partial protein sequence containing 335 amino acids (GenBank accession #AY642975). Locations of degenerate primers used for nested RT-PCR to obtain the initial cDNA are indicated in bold and with a dashed line with solid arrowhead. Locations of sequence-specific forward primers (cEcR F1 and cEcR F2) and degenerate nested reverse primer (EcR R4) used to obtain more of the 3' sequence are indicated in bold and with a dashed line with open arrowhead. Solid lines with solid arrowheads indicate locations of sequence-specific primers used for 5' RACE to obtain additional 5' sequence in the ORF (Table 1).