Fig. 4. The complete sequence of calpain T cDNA from land crab (Gl-CalpT). The
sequence (2639 bp) contained a full open reading frame (3042223 bp)
encoding a protein of 639 amino acids with a predicted mass of 74.56 kDa
(GenBank accession #AY639154). Locations of degenerate primer sites for
initial nested RT-PCR are underlined. Three amino acid residues comprising the
conserved catalytic triad (C, H, N) in domain II are in bold. The asterisk
indicates the stop codon. The poly(A) signal in the 3' UTR is in bold
and underlined.