Fig. 11. Tissue expression of land crab calpain B, M and T mRNAs using end-point RT-PCR. Total RNA from each tissue was DNase-treated, reverse-transcribed and PCR-amplified using primers specific for Calpains M, B and T (Table 2). Shown is a reversed image of an ethidium bromide-stained agarose gel of PCR products. The sizes of the products were 310 bp for CalpM, 538 bp for CalpB and 536 bp for CalpT. Gl-CalpB was expressed at varying levels in all tissues; Gl-CalpM was expressed in all tissues except Y-organ; Gl-CalpT was expressed in all tissues except eyestalk ganglia and Y-organ. Lane a, claw muscle (CM); b, leg muscle (LM); c, limb regenerate (LR); d, thoracic muscle (TM); e, gill (Gi); f, heart (Ht); g, hind gut (HG); h, thoracic ganglion (TG); i, eyestalk ganglia (EG); j, Y-organ (YO).