Fig. 7. Confocal fluoroscopy of Pd-mP emission spectra in digest cells in vitro. Digest cells were incubated for 4 h with Pd-mP-globin 100 µmol l^–1, and observed 8 h later using laser excitation at 514 nm. Images were collected from 530 to 700 nm, with ±10 nm intervals between each consecutive image. (A) Digest cell total emission image. Arrows indicate areas of the image selected for spectral analysis: (1) cytosol, (2) hemosome and (3) digest vesicle. Scale bar, 20 µm. (B) Emission spectra obtained from image analyses. (C) Pd-mP 5 µmol l^–1 fluorescence spectra with _ex=514 nm in water, methanol, phosphatidylcholine liposomes, or bound to 5 µmol l^–1 globin.