Fig. 4. Time course of proteolytic digestion of Pd-mP-globin. Fluorescence emission of Pd-mP (5 µmol l^–1) in water was recorded at 545 nm and 665 nm with excitation at 400 nm. Emission spectra A–D (insets) using the same excitation wavelength were acquired at the times indicated by black arrows, after addition of 5 µmol l^–1 globin (asterisks) and 1% bovine trypsin (red arrow).