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Fig. 5. The brightness test showed that the choice between the horizontal and the vertical plane of polarized light was made based on the orientation of the e-vectors, independent of the light intensity of the stimuli (80 trials per fish, N=4). Each circle represents the percentage of correct choice frequency (mean ± S.E.M.), when 0° was dimmer than 90°, whereas each square represents the percentage of correct choice frequency (mean ± S.E.M.) when 90° was dimmer than 0°. The horizontal shaded bands represent the range of choice frequency values that correspond to significance levels of P=0.001≤x and P=0.01≤x≤0.001, calculated using the binomial probability function for 40 Bernoulli trials. The reference e-vector for fish A and C was 90°, whereas the reference e-vector for fish B and D was 0°.