Fig. 1. mAb 313, which selectively recognizes
3-nAChRs, shows a pattern of
immunoreactivity very similar to that exhibited by mAb 210 in permeabilized
tissue. (A) Submucous ganglion stained with mAb 210 (17 nmol
l1) and FITC-goat-anti-rat (1:1000). Scale bar, 25 µm.
(B) Submucous ganglion stained with mAb 313 (35 nmol l1) and
FITC-goat-anti-rat (1:1000). Scale bar, 20 µm. In both preparations, the
tissue was permeabilized with 0.5% Triton X-100.