Fig. 9. Developmental time course of the relative abundance of three developmental stages – dechorionated encysted embryos (open diamond), emergence and emergence 2 stages combined (solid triangle) and nauplii (solid circle) – calculated as a percentage of all individuals present. Dechorionated embryos incubated in 35 Instant Ocean + 0.5% ethanol served as a control (A). Experimental treatments with 0.5 (B), 1.0 (C), 2.0 (D) and 4.0 µmol l^–1 (E) bafilomycin in the presence of 0.5% ethanol began at hour 0. To determine whether or not embryos were permeable to bafilomycin prior to emergence, 4 µmol l^–1 bafilomycin was added at hour 8 (F), as indicated with a broken line.