Fig. 1. (A) Alignment of the deduced amino acid sequence for the V-ATPase B-subunit from Artemia franciscana with mammalian brain-type (B2) isoforms (common name and GenBank accession numbers as follows: human, CAA44721 bovine, P31408; mouse, P50517; rat, NP_476561). (B) Alignment of the deduced amino acid sequence for the V-ATPase B-subunit from A. franciscana with mammalian kidney-type (B1) isoforms (human, NP_001683; bovine, P31407; mouse, NP_598918; rat, XP_232119). Sequence showing no differences between any of the species is represented by dots. White text on a black background represents mammalian sequence differing from A. franciscana but conserved among at least three of the four mammalian species (low variability). Black text on a blue background represents mammalian sequence differing both from Artemia and at least two mammalian sequences (high variability). Bold text on white background represents mammalian sequence with physiochemical properties similar to those of Artemia (potentially conserved). Gaps in the mammalian sequence are represented by a black dash on a grey background. The percent amino acid identity between aligned mammalian and A. franciscana sequence is enclosed in parentheses at the end of each sequence. Conserved NtpB domain used in phylogenetic analysis is bracketed by black and yellow arrow symbols above the A. franciscana sequence.