Fig. 5. In vivo real-time measurements of the heat shock response of mature fowl of three strains that differ in their resistance to heat. (A) Body temperature (T_b) regulation (N=3 in each strain). (B) HSF–DNA-binding activity detected by EMSA. EMSA was performed with a [³²P]HSE oligonucleotide and whole blood cell extracts. (C) Levels of HSP70–mRNA in whole blood cells, detected by chicken HSP70 cDNA as a probe. (D) Protein levels of HSP70 detected by western blot analysis. Leghorn-C, heat sensitive fowl; Leghorn-16d, phenotypically heat-adapted fowl; Bedouin, genetically heat-resistant fowl. Red arrows indicate repeated elevations of ambient temperature, in the case of the Bedouin fowl up to 43.5°C. B–D represent three individuals of each strain.