Fig. 3. A typical HSP response of postnatals (1 day, brain tissue) exposed to 6 h or 24 h at 42°C (N=6 in each treatment). (A) HSF–DNA-binding activity detected by EMSA. EMSA was performed with a [³²P]HSE oligonucleotide and whole brain tissue extracts. Western blot analysis of HSF1 and HSF3 (B) or HSP70 and HSP90 (C). Each lane represents a different individual. C, control, unexposed individuals; HS, at the end of the heat exposure. A similar pattern was observed up to 25 days of age. (D) In vivo metabolic labelling analyzed by [³⁵S]methionine incorporation. T_a, ambient temperature; T_b, body temperature.