Fig. 7. Cardioprotective influence of the mitoK_ATP opener diazoxide (50 µmol l^–1) and the stable cGMP analog 8-Br-cGMP (200 µmol l^–1) on the hypoxia-induced damage incurred by isolated cells. This figure illustrates the percentage change in the total fraction of stained cells after 60 min hypoxia in the presence of various agents, compared to that recorded after hypoxia alone. Under hypoxic conditions, both diazoxide and 8-Br-cGMP decreased the percentage of stained cells vs that seen with hypoxia alone. Blockade of mitoK_ATP channel activity with 5-HD (100 µmol l^–1) reduced but did not eliminate the beneficial effect of diazoxide, and did not affect the response to 8-Br-cGMP.