Fig. 5. Effect of 60 min hypoxia in vitro on the incidence of cell staining by Trypan Blue. Under hypoxic conditions, a significantly greater percentage of cells was unable to exclude the stain compared to that observed in normoxia, suggesting cellular injury. A total of more than more than 500 myocytes were individually evaluated during six experiments; values are mean changes ± 1 S.E.M. ***Significantly different from values recorded in normoxia (P<0.001).