JEB -- Scott et al. 208 (14): 2719 Figure IG2

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Fig. 2. The estimated absolute expression levels of several genes important for ion transport in the gills (black bars) and opercular epithelium (grey bars) of killifish (N=15–20). Values are determined using Formula 1 and data are normalized to the expression level of elongation factor 1 ${alpha}$ (EF1 ${alpha}$ ). Na⁺,H⁺-exchanger 2 (NHE2) was not amplified above background levels in opercular epithelium. NKA, Na⁺,K⁺-ATPase ${alpha}$ _1a; VHA, V-type H⁺-ATPase A. ^#Significant difference between tissues (P<0.05).