JEB -- Watanabe et al. 208 (14): 2673 Figure IG6

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 6. Double immunofluorescence microscopy of gills in tilapia adapted to freshwater (FW; A,C,E,G) and seawater (SW; B,D,F,H), stained with anti-tilapia AQP3 (A,B) and anti-Na⁺/K⁺-ATPase as a marker of chloride cells (C,D). E and F are merged images of A,C and B,D, respectively. AQP3-immunoreactive cells are observed in both FW and SW gills (A,B), and immunoreactions for AQP3 and Na⁺/K⁺-ATPase coincided completely with each other (E,F). No immunoreaction is observed in control sections (G,H). Bar, 50 µm.