Fig. 5. Western blot analysis with an antibody raised against a synthetic peptide corresponding to part of the C-terminal region of tilapia AQP3. After immunoprecipitation with anti-tilapia AQP3, the gill protein sample was separated by SDS-polyacrylamide gel electrophoresis and transferred to a membrane. Immunoreactive bands were detected by the ABC method. Positions of molecular mass markers (kDa) are shown on the left.