Fig. 1. The hysteresis freezing points of the gastrointestinal fluids and serum from Arctogadus glacialis that had ingested Boreogadus saida (Bs) and crustaceans (Cr). The dark gray boxes represent the melting point depression due to measured ions, light gray boxes represent the difference between the measured melting point and the melting point depression due to measured ions. The open boxes represent the thermal hysteresis due to AFGP. Data are given as mean ± S.E.M. Statistical significance was tested at the P0.05 significance level by using a two-tailed Student's t-test. The food type only had significant influence on the antifreeze activity of the mid-gut fluids.