Fig. 3. LANB2 mRNA accumulation in the midgut. Semi-quantitative PCR was
performed to determine LANB2 mRNA accumulation in isolated midguts
(Lam) using S7 mRNA as a standard (S7). 1, sugarfed
mosquitoes injected with dsRNA against GFP; 2, sugar-fed mosquitoes injected
with dsRNA against LANB2; 3, blood-fed mosquitoes injected with dsRNA
against GFP; 4, blood-fed mosquitoes injected with dsRNA against
LANB2. See Materials and methods for experimental details.