Fig. 2. (A) Schematic representation of the Anopheles gambiae LANB2 gene. The coding area is represented by boxes corresponding to the domains of the protein (grey: domain V, containing EGF repeats and shown previously to interact with P25 and P28). The black line indicates non-coding segments, while the small map below shows the extent of the segment used to obtain the dsRNA used in the experiments spanning the nucleotides indicated. (B) Effects of dsRNA injections. The bars indicate the mean number of oocysts found in the midguts of mosquitoes that had previously been injected with dsRNA directed against the GFP gene as a control (a) or the LANB2 gene (b). Error bars represent standard error of the mean (N=85 and N=79, respectively). The asterisk indicates a significant difference in oocyst burden (P<0.0001). See Results for details. (C) Estimates of the LANB2 gene mRNA copy number expressed in percent of the S7 gene mRNA copies after injection of dsRNA determined by quantitative real-time PCR. a, sugar-fed mosquitoes injected with dsRNA against GFP; b, sugar-fed mosquitoes injected with dsRNA against LANB2; c, blood-fed mosquitoes injected with dsRNA against GFP; d, bloodfed mosquitoes injected with dsRNA against LANB2. Error bars represent the standard error of the mean (N=3). See Materials and methods for experimental details.