Fig. 2. Cryptocyanin and hemocyanin mRNA expression in C. magister
hepatopancreas during one molt cycle. Fifth-instar juvenile cohort, ecdysis on
day 0 and day 28. Real-time quantitative PCR data from three target mRNAs:
cryptocyanin, hemocyanin and tubulin. mRNA levels of cryptocyanin and
hemocyanin were normalized to that of tubulin mRNA, which was constant
throughout the molt cycle. (A) Hemocyanin mRNA/tubulin mRNA (x); (B)
cryptocyanin mRNA/tubulin mRNA (x), hemocyanin mRNA/tubulin mRNA
(x). Note that the y-axis scale in B is 10-fold greater than in
A. Two crabs sampled per time point.