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Fig. 2. Cryptocyanin and hemocyanin mRNA expression in C. magister hepatopancreas during one molt cycle. Fifth-instar juvenile cohort, ecdysis on day 0 and day 28. Real-time quantitative PCR data from three target mRNAs: cryptocyanin, hemocyanin and tubulin. mRNA levels of cryptocyanin and hemocyanin were normalized to that of tubulin mRNA, which was constant throughout the molt cycle. (A) Hemocyanin mRNA/tubulin mRNA (x); (B) cryptocyanin mRNA/tubulin mRNA (x), hemocyanin mRNA/tubulin mRNA (x). Note that the y-axis scale in B is 10-fold greater than in A. Two crabs sampled per time point.